In silico design and bioinformatics guides
Practical, evergreen explainers on the computational design and analysis behind molecular biology, from assay and primer design to expression-vector and CRISPR guide design. Written by the team building an autonomous AI in silico designer.
What is an AI in silico designer for molecular biology?
An AI in silico designer autonomously designs primers, probes, CRISPR guides and DNA constructs and runs the bioinformatics behind lab experiments, with human review.
AI in silico design vs traditional bioinformatics (CRO, freelancer, ChatGPT)
How an AI in silico designer compares with a CRO, a freelance bioinformatician, and ChatGPT for molecular biology design: turnaround, reproducibility, cost, and QC.
How to design a ddPCR assay in silico
A step-by-step in silico workflow for ddPCR assay design: target selection, primer and probe parameters, specificity checking, multiplexing, and common pitfalls.
In silico expression-vector design for CHO cells
An in silico workflow for designing a CHO cell expression vector: backbone elements, promoter choice, codon optimization, signal peptide, selection system, and QC.
Primer and probe design: best practices for qPCR, RT-qPCR, and ddPCR
Best practices for designing PCR primers and hydrolysis probes: melting temperature, GC content, amplicon length, specificity checking, dimers, and RT-qPCR intron spanning.
CRISPR guide RNA (sgRNA) design in silico: a practical workflow
An in silico workflow for CRISPR sgRNA design: target and PAM selection, on-target activity scoring, off-target prediction, cross-tool validation, and modality notes.